› 11gs

X-ray diffraction
2.3Å resolution

Glutathione s-transferase complexed with ethacrynic acid-glutathione conjugate (form ii)

Released:
DOI: 10.2210/pdb11gs/pdb
PDB entry 11gs coloured by chain, front view.
PDB entry 11gs coloured by chain, side view.
PDB entry 11gs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The glutathione conjugate of ethacrynic acid can bind to human pi class glutathione transferase P1-1 in two different modes.
Oakley AJ, Lo Bello M, Mazzetti AP, Federici G, Parker MW
FEBS Lett 419 32-6 (1997)
PMID: 9426214

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase P Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 23.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09211 (Residues: 1-210; Coverage: 100%)
Gene names: FAEES3, GST3, GSTP1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
2 bound ligands:
Ligand EAA 2 x EAA
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 77.78Å b: 89.56Å c: 68.65Å
α: 90° β: 97.62° γ: 90°
R-values:
R R work R free
0.214 0.214 0.27
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The role of glutathione-S-transferase in anti-cancer drug resistance.
Townsend et al. (2003)
4 more

14 mentions without citation

Exploring off-targets and off-systems for adverse drug reactions via chemical-protein interactome--clozapine-induced agranulocytosis as a case study.
Yang et al. (2011)
13 more