› 1a14

X-ray diffraction
2.5Å resolution

COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE

Released:
DOI: 10.2210/pdb1a14/pdb
PDB entry 1a14 coloured by chain, front view.
PDB entry 1a14 coloured by chain, side view.
PDB entry 1a14 coloured by chain, top view.
Source organisms:
Primary publication:
Three-dimensional structures of single-chain Fv-neuraminidase complexes.
Malby RL, McCoy AJ, Kortt AA, Hudson PJ, Colman PM
J Mol Biol 279 901-10 (1998)
PMID: 9642070

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-134473 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
Neuraminidase Chain: N
Molecule details ›
Chain: N
Length: 388 amino acids
Theoretical weight: 43.72 KDa
Source organism: Influenza A virus
UniProt:
  • Canonical: P03472 (Residues: 83-470; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase
Ig heavy chain V region 3 Chain: H
Molecule details ›
Chain: H
Length: 120 amino acids
Theoretical weight: 13.23 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01749 (Residues: 20-117; Coverage: 100%)
Gene names: Igh-VJ558, Ighv1-61
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig kappa chain V-V region HP 93G7 Chain: L
Molecule details ›
Chain: L
Length: 104 amino acids
Theoretical weight: 11.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01645 (Residues: 1-102; Coverage: 94%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN, NAG, BMA
Carbohydrate polymer
3 bound ligands:
Ligand MAN 1 x MAN
Ligand NAG 2 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: I422
Unit cell:
a: 165.3Å b: 165.3Å c: 182.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.27
Expression system: Escherichia coli

Quick links

Citations

3 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
2 more

17 mentions without citation

Function and 3D structure of the N-glycans on glycoproteins.
Nagae et al. (2012)
16 more