› 1a2b

X-ray diffraction
2.4Å resolution

HUMAN RHOA COMPLEXED WITH GTP ANALOGUE

Released:
DOI: 10.2210/pdb1a2b/pdb
PDB entry 1a2b coloured by chain, front view.
PDB entry 1a2b coloured by chain, side view.
PDB entry 1a2b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue.
Ihara K, Muraguchi S, Kato M, Shimizu T, Shirakawa M, Kuroda S, Kaibuchi K, Hakoshima T
J Biol Chem 273 9656-66 (1998)
PMID: 9545299

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61586 (Residues: 1-181; Coverage: 94%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GSP 1 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 62.02Å b: 74.78Å c: 50.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.268
Expression system: Escherichia coli BL21

Quick links

Citations

17 review citations

Rho GTPases and their effector proteins.
Bishop et al. (2000)
16 more

39 mentions without citation

Redox regulation of Ras and Rho GTPases: mechanism and function.
Mitchell et al. (2013)
38 more