› 1a3q

X-ray diffraction
2.1Å resolution

HUMAN NF-KAPPA-B P52 BOUND TO DNA

Released:
DOI: 10.2210/pdb1a3q/pdb
PDB entry 1a3q coloured by chain, front view.
PDB entry 1a3q coloured by chain, side view.
PDB entry 1a3q coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human NF-kappaB p52 homodimer-DNA complex at 2.1 A resolution.
Cramer P, Larson CJ, Verdine GL, Müller CW
EMBO J 16 7078-90 (1997)
PMID: 9384586

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113392 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear factor NF-kappa-B p52 subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 285 amino acids
Theoretical weight: 32.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00653 (Residues: 37-327; Coverage: 32%)
Gene names: LYT10, NFKB2
Sequence domains:
Structure domains:
DNA (5'-D(*GP*GP*GP*GP*AP*AP*TP*CP*CP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*GP*GP*GP*AP*TP*TP*CP*CP*CP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 44.2Å b: 121Å c: 134.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.32
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

Transcriptional regulation via the NF-kappaB signaling module.
Hoffmann et al. (2006)
12 more

32 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
31 more