› 1a4i

X-ray diffraction
1.5Å resolution

HUMAN TETRAHYDROFOLATE DEHYDROGENASE / CYCLOHYDROLASE

Released:
DOI: 10.2210/pdb1a4i/pdb
PDB entry 1a4i coloured by chain, front view.
PDB entry 1a4i coloured by chain, side view.
PDB entry 1a4i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The 3-D structure of a folate-dependent dehydrogenase/cyclohydrolase bifunctional enzyme at 1.5 A resolution.
Allaire M, Li Y, MacKenzie RE, Cygler M
Structure 6 173-82 (1998)
PMID: 9519408

Function and Biology Details

Reactions catalysed: 
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 32.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11586 (Residues: 1-301; Coverage: 32%)
Gene names: MTHFC, MTHFD, MTHFD1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 67.523Å b: 135.843Å c: 61.403Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.235
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Tetrahydrofolate and tetrahydromethanopterin compared: functionally distinct carriers in C1 metabolism.
Maden BE. (2000)
38 other articles

16 mentions without citation

A Review of Small-Molecule Inhibitors of One-Carbon Enzymes: SHMT2 and MTHFD2 in the Spotlight.
Cuthbertson et al. (2021)
15 more