› 1a4r

X-ray diffraction
2.5Å resolution

G12V MUTANT OF HUMAN PLACENTAL CDC42 GTPASE IN THE GDP FORM

Released:
DOI: 10.2210/pdb1a4r/pdb
PDB entry 1a4r coloured by chain, front view.
PDB entry 1a4r coloured by chain, side view.
PDB entry 1a4r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nucleotide binding to the G12V-mutant of Cdc42 investigated by X-ray diffraction and fluorescence spectroscopy: two different nucleotide states in one crystal.
Rudolph MG, Wittinghofer A, Vetter IR
Protein Sci 8 778-87 (1999)
PMID: 10211824

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division control protein 42 homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P60953 (Residues: 1-191; Coverage: 100%)
Gene name: CDC42
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand GDP 1 x GDP
Ligand GNH 1 x GNH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P41212
Unit cell:
a: 98.65Å b: 98.65Å c: 104.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

SH3 domains: modules of protein-protein interactions.
Kurochkina et al. (2013)
1 more

12 mentions without citation

Structural biology and bioinformatics in drug design: opportunities and challenges for target identification and lead discovery.
Blundell et al. (2006)
11 more