› 1a6y

X-ray diffraction
2.3Å resolution

REVERBA ORPHAN NUCLEAR RECEPTOR/DNA COMPLEX

Released:
DOI: 10.2210/pdb1a6y/pdb
PDB entry 1a6y coloured by chain, front view.
PDB entry 1a6y coloured by chain, side view.
PDB entry 1a6y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural elements of an orphan nuclear receptor-DNA complex.
Zhao Q, Khorasanizadeh S, Miyoshi Y, Lazar MA, Rastinejad F
Mol Cell 1 849-61 (1998)
PMID: 9660968

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113396 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nuclear receptor subfamily 1 group D member 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 94 amino acids
Theoretical weight: 10.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P20393 (Residues: 123-216; Coverage: 15%)
Gene names: EAR1, HREV, NR1D1, THRAL
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*AP*AP*CP*TP*AP*GP*GP*TP*CP*AP*CP*(5IT)P*AP*GP*GP*TP*CP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.25 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*TP*GP*AP*CP*CP*TP*AP*GP*TP*GP*AP*CP*CP*TP*AP*GP*TP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
1 modified residue:
Ligand 5IU 1 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P1
Unit cell:
a: 38.67Å b: 45.69Å c: 47.95Å
α: 75.16° β: 80.64° γ: 85.58°
R-values:
R R work R free
0.192 0.192 0.288
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

23 review citations

The mechanism of action of thyroid hormones.
Zhang et al. (2000)
22 more

8 mentions without citation

Treble clef finger--a functionally diverse zinc-binding structural motif.
Grishin NV. (2001)
7 more