› 1a81

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF THE TANDEM SH2 DOMAIN OF THE SYK KINASE BOUND TO A DUALLY TYROSINE-PHOSPHORYLATED ITAM

Released:
DOI: 10.2210/pdb1a81/pdb
PDB entry 1a81 coloured by chain, front view.
PDB entry 1a81 coloured by chain, side view.
PDB entry 1a81 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Syk tyrosine kinase ubiquity in signal transduction pathways revealed by the crystal structure of its regulatory SH2 domains bound to a dually phosphorylated ITAM peptide.
Fütterer K, Wong J, Grucza RA, Chan AC, Waksman G
J Mol Biol 281 523-37 (1998)
PMID: 9698567

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139750 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase SYK Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 254 amino acids
Theoretical weight: 28.83 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43405 (Residues: 9-262; Coverage: 40%)
Gene name: SYK
Sequence domains: SH2 domain
Structure domains:
T-cell surface glycoprotein CD3 epsilon chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 18 amino acids
Theoretical weight: 2.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P07766 (Residues: 186-203; Coverage: 10%)
Gene names: CD3E, T3E
Sequence domains: Immunoreceptor tyrosine-based activation motif

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 12 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 85.5Å b: 146.9Å c: 91.5Å
α: 90° β: 97.6° γ: 90°
R-values:
R R work R free
0.226 0.226 0.317
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

39 review citations

The SYK tyrosine kinase: a crucial player in diverse biological functions.
Mócsai et al. (2010)
38 more

20 mentions without citation

ZAP-70: an essential kinase in T-cell signaling.
Wang et al. (2010)
19 more