› 1a8m

X-ray diffraction
2.3Å resolution

TUMOR NECROSIS FACTOR ALPHA, R31D MUTANT

Released:
DOI: 10.2210/pdb1a8m/pdb
PDB entry 1a8m coloured by chain, front view.
PDB entry 1a8m coloured by chain, side view.
PDB entry 1a8m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of TNF-alpha mutant R31D with greater affinity for receptor R1 compared with R2.
Reed C, Fu ZQ, Wu J, Xue YN, Harrison RW, Chen MJ, Weber IT
Protein Eng 10 1101-7 (1997)
PMID: 9488135

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tumor necrosis factor, soluble form Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 157 amino acids
Theoretical weight: 17.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01375 (Residues: 77-233; Coverage: 67%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P41212
Unit cell:
a: 94.7Å b: 94.7Å c: 117.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.24
Expression system: Escherichia coli

Quick links

Citations

1 review citation

TNF alpha and the TNF receptor superfamily: structure-function relationship(s).
Idriss et al. (2000)
19 other articles

22 mentions without citation

Prediction of functional sites by analysis of sequence and structure conservation.
Panchenko et al. (2004)
21 more