› 1ad5

X-ray diffraction
2.6Å resolution

SRC FAMILY KINASE HCK-AMP-PNP COMPLEX

Released:
DOI: 10.2210/pdb1ad5/pdb
PDB entry 1ad5 coloured by chain, front view.
PDB entry 1ad5 coloured by chain, side view.
PDB entry 1ad5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the Src family tyrosine kinase Hck.
Sicheri F, Moarefi I, Kuriyan J
Nature 385 602-9 (1997)
PMID: 9024658

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein kinase HCK Chains: A, B
Molecule details ›
Chains: A, B
Length: 438 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P08631 (Residues: 79-526; Coverage: 83%)
Gene name: HCK
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand ANP 2 x ANP
1 modified residue:
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 72.767Å b: 92.36Å c: 178.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.307
Expression system: Spodoptera frugiperda

Quick links

Citations

163 review citations

Free radicals in the physiological control of cell function.
Dröge W. (2002)
162 more

18 mentions without citation

Structure and dynamic regulation of Src-family kinases.
Engen et al. (2008)
17 more