› 1adq

X-ray diffraction
3.15Å resolution

CRYSTAL STRUCTURE OF A HUMAN IGM RHEUMATOID FACTOR FAB IN COMPLEX WITH ITS AUTOANTIGEN IGG FC

Released:
DOI: 10.2210/pdb1adq/pdb
PDB entry 1adq coloured by chain, front view.
PDB entry 1adq coloured by chain, side view.
PDB entry 1adq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human IgM rheumatoid factor Fab bound to its autoantigen IgG Fc reveals a novel topology of antibody-antigen interaction.
Corper AL, Sohi MK, Bonagura VR, Steinitz M, Jefferis R, Feinstein A, Beale D, Taussig MJ, Sutton BJ
Nat Struct Biol 4 374-81 (1997)
PMID: 9145108

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-224985 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Immunoglobulin heavy constant gamma 4 Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 23.48 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01861 (Residues: 118-323; Coverage: 52%)
Gene name: IGHG4
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
IGM-LAMBDA RF-AN FAB (LIGHT CHAIN) Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 22.57 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
IGM-LAMBDA RF-AN FAB (HEAVY CHAIN) Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 24.67 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 160.35Å b: 81.95Å c: 64.24Å
α: 90° β: 98.27° γ: 90°
R-values:
R R work R free
0.225 0.225 0.289

Quick links

Citations

27 review citations

IgG4 breaking the rules.
Aalberse et al. (2002)
26 more

23 mentions without citation

Intramolecular immunological signal hypothesis revived--structural background of signalling revealed by using Congo Red as a specific tool.
Jagusiak et al. (2015)
22 more