› 1aka

X-ray diffraction
2.1Å resolution

STRUCTURAL BASIS FOR THE CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE K258H LACKING ITS PYRIDOXAL-5'-PHOSPHATE-BINDING LYSINE RESIDUE

Released:
DOI: 10.2210/pdb1aka/pdb
PDB entry 1aka coloured by chain, front view.
PDB entry 1aka coloured by chain, side view.
PDB entry 1aka coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis for the catalytic activity of aspartate aminotransferase K258H lacking the pyridoxal 5'-phosphate-binding lysine residue.
Malashkevich VN, Jäger J, Ziak M, Sauder U, Gehring H, Christen P, Jansonius JN
Biochemistry 34 405-14 (1995)
PMID: 7819232

Function and Biology Details

Reactions catalysed: 
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132574 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aspartate aminotransferase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 401 amino acids
Theoretical weight: 45 KDa
Source organism: Gallus gallus
Expression system: unidentified
UniProt:
  • Canonical: P00508 (Residues: 23-423; Coverage: 95%)
Gene name: GOT2
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 55.87Å b: 58.8Å c: 75.81Å
α: 85.26° β: 108.96° γ: 115.57°
R-values:
R R work R free
0.169 not available not available
Expression system: unidentified

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Citations

10 citation in other articles

The crystal structure of methenyltetrahydromethanopterin cyclohydrolase from the hyperthermophilic archaeon Methanopyrus kandleri.
Grabarse et al. (1999)
9 more

2 mentions without citation

A comprehensive analysis of the structure-function relationship in proteins based on local structure similarity.
Hvidsten et al. (2009)
1 more