› 1akj

X-ray diffraction
2.65Å resolution

COMPLEX OF THE HUMAN MHC CLASS I GLYCOPROTEIN HLA-A2 AND THE T CELL CORECEPTOR CD8

Released:
DOI: 10.2210/pdb1akj/pdb
PDB entry 1akj coloured by chain, front view.
PDB entry 1akj coloured by chain, side view.
PDB entry 1akj coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the complex between human CD8alpha(alpha) and HLA-A2.
Gao GF, Tormo J, Gerth UC, Wyer JR, McMichael AJ, Stuart DI, Bell JI, Jones EY, Jakobsen BK
Nature 387 630-4 (1997)
PMID: 9177355

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134577 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
HIV REVERSE TRANSCRIPTASE EPITOPE Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 993 Da
Source organism: Human immunodeficiency virus
Expression system: Not provided
T-cell surface glycoprotein CD8 alpha chain Chains: D, E
Molecule details ›
Chains: D, E
Length: 120 amino acids
Theoretical weight: 13.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01732 (Residues: 22-141; Coverage: 56%)
Gene names: CD8A, MAL
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 70.9Å b: 89.6Å c: 116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

76 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
75 more

62 mentions without citation

The T cell antigen receptor: the Swiss army knife of the immune system.
Attaf et al. (2015)
61 more