› 1ao7

X-ray diffraction
2.6Å resolution

COMPLEX BETWEEN HUMAN T-CELL RECEPTOR, VIRAL PEPTIDE (TAX), AND HLA-A 0201

Released:
DOI: 10.2210/pdb1ao7/pdb
PDB entry 1ao7 coloured by chain, front view.
PDB entry 1ao7 coloured by chain, side view.
PDB entry 1ao7 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the complex between human T-cell receptor, viral peptide and HLA-A2.
Garboczi DN, Ghosh P, Utz U, Fan QR, Biddison WE, Wiley DC
Nature 384 134-41 (1996)
PMID: 8906788

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero decamer
PDBe Complex ID:
PDB-CPX-100460 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Protein Tax-1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.07 KDa
Source organism: Human T-cell leukemia virus type I
Expression system: Not provided
UniProt:
  • Canonical: P14079 (Residues: 11-19; Coverage: 3%)
Gene name: tax
T cell receptor alpha chain constant; T cell receptor alpha variable 12-2 Chain: D
Molecule details ›
Chain: D
Length: 204 amino acids
Theoretical weight: 22.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A075B6T6 (Residues: 23-112; Coverage: 97%)
  • Canonical: P01848 (Residues: 1-93; Coverage: 66%)
Gene names: TCRA, TRAC, TRAV12-2
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 2; T cell receptor beta variable 6-5 Chain: E
Molecule details ›
Chain: E
Length: 245 amino acids
Theoretical weight: 27.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0K0K1A5 (Residues: 20-114; Coverage: 100%)
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TRBC2, TRBV6-5
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand EMC 2 x EMC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2
Unit cell:
a: 229.3Å b: 49.5Å c: 96Å
α: 90° β: 89.6° γ: 90°
R-values:
R R work R free
0.245 0.245 0.32
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

223 review citations

How TCRs bind MHCs, peptides, and coreceptors.
Rudolph et al. (2006)
222 more

79 mentions without citation

The molecular basis of TCR germline bias for MHC is surprisingly simple.
Garcia et al. (2009)
78 more