› 1as2

X-ray diffraction
2.8Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.31 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10824 (Residues: 2-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P43212
Unit cell:
a: 77.19Å b: 77.19Å c: 143.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins.
Ross et al. (2000)
8 more

4 mentions without citation

A novel DNA enzyme reduces glycosaminoglycan chains in the glial scar and allows microtransplanted dorsal root ganglia axons to regenerate beyond lesions in the spinal cord.
Grimpe et al. (2004)
3 more