› 1ayp

X-ray diffraction
2.57Å resolution

A PROBE MOLECULE COMPOSED OF SEVENTEEN PERCENT OF TOTAL DIFFRACTING MATTER GIVES CORRECT SOLUTIONS IN MOLECULAR REPLACEMENT

Released:
DOI: 10.2210/pdb1ayp/pdb
PDB entry 1ayp coloured by chain, front view.
PDB entry 1ayp coloured by chain, side view.
PDB entry 1ayp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A probe molecule composed of seventeen percent of total diffracting matter gives correct solutions in molecular replacement.
Oh BH
Acta Crystallogr D Biol Crystallogr 51 140-4 (1995)
PMID: 15299314

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146965 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2, membrane associated Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 124 amino acids
Theoretical weight: 13.95 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P14555 (Residues: 21-144; Coverage: 100%)
Gene names: PLA2B, PLA2G2A, PLA2L, RASF-A
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 12 x CA
Ligand INB 6 x INB
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 64.51Å b: 114.72Å c: 64.43Å
α: 90° β: 120.13° γ: 90°
R-values:
R R work R free
0.214 0.214 not available
Expression system: Not provided

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Citations

1 review citation

Modern developments in molecular replacement.
Turkenburg et al. (1996)
2 other articles

5 mentions without citation

Pro-inflammatory secretory phospholipase A2 type IIA binds to integrins alphavbeta3 and alpha4beta1 and induces proliferation of monocytic cells in an integrin-dependent manner.
Saegusa et al. (2008)
4 more