› 1b3e

X-ray diffraction
2.5Å resolution

HUMAN SERUM TRANSFERRIN, N-TERMINAL LOBE, EXPRESSED IN PICHIA PASTORIS

Released:
DOI: 10.2210/pdb1b3e/pdb
PDB entry 1b3e coloured by chain, front view.
PDB entry 1b3e coloured by chain, side view.
PDB entry 1b3e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32.
Bewley MC, Tam BM, Grewal J, He S, Shewry S, Murphy ME, Mason AB, Woodworth RC, Baker EN, MacGillivray RT
Biochemistry 38 2535-41 (1999)
PMID: 10029548

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136222 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serotransferrin Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.51 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P02787 (Residues: 23-352; Coverage: 49%)
Gene names: PRO1400, TF
Sequence domains: Transferrin
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand CO3 1 x CO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 73.5Å b: 73.5Å c: 156.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.255
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Heterologous protein expression in the methylotrophic yeast Pichia pastoris.
Cereghino et al. (2000)
1 more

1 mention without citation

Predicted structural mimicry of spike receptor-binding motifs from highly pathogenic human coronaviruses.
Beaudoin et al. (2021)