› 1b3u

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF CONSTANT REGULATORY DOMAIN OF HUMAN PP2A, PR65ALPHA

Released:
DOI: 10.2210/pdb1b3u/pdb
PDB entry 1b3u coloured by chain, front view.
PDB entry 1b3u coloured by chain, side view.
PDB entry 1b3u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the protein phosphatase 2A PR65/A subunit reveals the conformation of its 15 tandemly repeated HEAT motifs.
Groves MR, Hanlon N, Turowski P, Hemmings BA, Barford D
Cell 96 99-110 (1999)
PMID: 9989501

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chains: A, B
Molecule details ›
Chains: A, B
Length: 588 amino acids
Theoretical weight: 65.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30153 (Residues: 2-589; Coverage: 100%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 64.28Å b: 112.15Å c: 122.63Å
α: 90° β: 95.6° γ: 90°
R-values:
R R work R free
0.209 0.209 0.268
Expression system: Escherichia coli

Quick links

Citations

73 review citations

TOR, a central controller of cell growth.
Schmelzle et al. (2000)
72 more

31 mentions without citation

Folding cooperativity and allosteric function in the tandem-repeat protein class.
Perez-Riba et al. (2018)
30 more