› 1b72

X-ray diffraction
2.35Å resolution

PBX1, HOMEOBOX PROTEIN HOX-B1/DNA TERNARY COMPLEX

Released:
DOI: 10.2210/pdb1b72/pdb
PDB entry 1b72 coloured by chain, front view.
PDB entry 1b72 coloured by chain, side view.
PDB entry 1b72 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a HoxB1-Pbx1 heterodimer bound to DNA: role of the hexapeptide and a fourth homeodomain helix in complex formation.
Piper DE, Batchelor AH, Chang CP, Cleary ML, Wolberger C
Cell 96 587-97 (1999)
PMID: 10052460

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113464 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox protein Hox-B1 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 11.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14653 (Residues: 171-266; Coverage: 32%)
Gene names: HOX2I, HOXB1
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
Pre-B-cell leukemia transcription factor 1 Chain: B
Molecule details ›
Chain: B
Length: 87 amino acids
Theoretical weight: 10.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40424 (Residues: 233-319; Coverage: 20%)
Gene names: PBX1, PRL
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*AP*CP*TP*CP*TP*AP*TP*GP*AP*TP*TP*GP*AP*TP*CP*GP*GP*CP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
DNA (5'-D(*TP*CP*AP*GP*CP*CP*GP*AP*TP*CP*AP*AP*TP*CP*AP*TP*AP*GP*AP*G)-3') Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.13 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 58.67Å b: 64.58Å c: 81.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.277
Expression system: Escherichia coli

Quick links

Citations

37 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
36 more

34 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
33 more