› 1bi9

X-ray diffraction
2.7Å resolution

RETINAL DEHYDROGENASE TYPE TWO WITH NAD BOUND

Released:
DOI: 10.2210/pdb1bi9/pdb
PDB entry 1bi9 coloured by chain, front view.
PDB entry 1bi9 coloured by chain, side view.
PDB entry 1bi9 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
The structure of retinal dehydrogenase type II at 2.7 A resolution: implications for retinal specificity.
Lamb AL, Newcomer ME
Biochemistry 38 6003-11 (1999)
PMID: 10320326

Function and Biology Details

Reaction catalysed: 
Retinal + NAD(+) + H(2)O = retinoate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-179030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinal dehydrogenase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 499 amino acids
Theoretical weight: 54.8 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q63639 (Residues: 20-518; Coverage: 96%)
Gene names: Aldh1a2, Raldh2
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21212
Unit cell:
a: 149.8Å b: 167.3Å c: 107.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.289
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily.
Marchitti et al. (2008)
7 more

10 mentions without citation

Aldehyde dehydrogenases: from eye crystallins to metabolic disease and cancer stem cells.
Vasiliou et al. (2013)
9 more