› 1bix

X-ray diffraction
2.2Å resolution

THE CRYSTAL STRUCTURE OF THE HUMAN DNA REPAIR ENDONUCLEASE HAP1 SUGGESTS THE RECOGNITION OF EXTRA-HELICAL DEOXYRIBOSE AT DNA ABASIC SITES

Released:
DOI: 10.2210/pdb1bix/pdb
PDB entry 1bix coloured by chain, front view.
PDB entry 1bix coloured by chain, side view.
PDB entry 1bix coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the human DNA repair endonuclease HAP1 suggests the recognition of extra-helical deoxyribose at DNA abasic sites.
Gorman MA, Morera S, Rothwell DG, de La Fortelle E, Mol CD, Tainer JA, Hickson ID, Freemont PS
EMBO J 16 6548-58 (1997)
PMID: 9351835

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair nuclease/redox regulator APEX1 Chain: A
Molecule details ›
Chain: A
Length: 287 amino acids
Theoretical weight: 32.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27695 (Residues: 32-318; Coverage: 90%)
Gene names: APE, APE1, APEX, APEX1, APX, HAP1, REF1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

2 bound ligands:
Ligand SM 4 x SM
Ligand PT 1 x PT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 87.26Å b: 44.701Å c: 78.778Å
α: 90° β: 103.45° γ: 90°
R-values:
R R work R free
0.184 0.184 0.269
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

44 review citations

Biology of mammalian L1 retrotransposons.
Ostertag et al. (2001)
43 more

26 mentions without citation

Redox regulation of DNA repair: implications for human health and cancer therapeutic development.
Luo et al. (2010)
25 more