› 1bj4

X-ray diffraction
2.65Å resolution

RECOMBINANT SERINE HYDROXYMETHYLTRANSFERASE (HUMAN)

Released:
DOI: 10.2210/pdb1bj4/pdb
PDB entry 1bj4 coloured by chain, front view.
PDB entry 1bj4 coloured by chain, side view.
PDB entry 1bj4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human cytosolic serine hydroxymethyltransferase: a target for cancer chemotherapy.
Renwick SB, Snell K, Baumann U
Structure 6 1105-16 (1998)
PMID: 9753690

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase, cytosolic Chain: A
Molecule details ›
Chain: A
Length: 470 amino acids
Theoretical weight: 51.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P34896 (Residues: 11-480; Coverage: 97%)
Gene name: SHMT1
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P6222
Unit cell:
a: 154.4Å b: 154.4Å c: 235.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.21 0.226
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Serine and glycine metabolism in cancer.
Amelio et al. (2014)
14 more

19 mentions without citation

A Review of Small-Molecule Inhibitors of One-Carbon Enzymes: SHMT2 and MTHFD2 in the Spotlight.
Cuthbertson et al. (2021)
18 more