› 1bk5

X-ray diffraction
2.2Å resolution

KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE

Released:
DOI: 10.2210/pdb1bk5/pdb
PDB entry 1bk5 coloured by chain, front view.
PDB entry 1bk5 coloured by chain, side view.
PDB entry 1bk5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha.
Conti E, Uy M, Leighton L, Blobel G, Kuriyan J
Cell 94 193-204 (1998)
PMID: 9695948

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169774 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Importin subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 422 amino acids
Theoretical weight: 46.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02821 (Residues: 89-510; Coverage: 78%)
Gene names: KAP60, N1606, SRP1, YNL189W
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

1 bound ligand:
Ligand CO 2 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 157.98Å b: 74.14Å c: 84.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

92 review citations

Transport between the cell nucleus and the cytoplasm.
Görlich et al. (1999)
91 more

14 mentions without citation

The G protein α chaperone Ric-8 as a potential therapeutic target.
Papasergi et al. (2015)
13 more