› 1bkc

X-ray diffraction
2Å resolution

CATALYTIC DOMAIN OF TNF-ALPHA CONVERTING ENZYME (TACE)

Released:
DOI: 10.2210/pdb1bkc/pdb
PDB entry 1bkc coloured by chain, front view.
PDB entry 1bkc coloured by chain, side view.
PDB entry 1bkc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the catalytic domain of human tumor necrosis factor-alpha-converting enzyme.
Maskos K, Fernandez-Catalan C, Huber R, Bourenkov GP, Bartunik H, Ellestad GA, Reddy P, Wolfson MF, Rauch CT, Castner BJ, Davis R, Clarke HR, Petersen M, Fitzner JN, Cerretti DP, March CJ, Paxton RJ, Black RA, Bode W
Proc Natl Acad Sci U S A 95 3408-12 (1998)
PMID: 9520379

Function and Biology Details

Reaction catalysed: 
Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha). Similarly cleaves other membrane-anchored, cell-surface proteins to 'shed' the extracellular domains.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160275 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Disintegrin and metalloproteinase domain-containing protein 17 Chains: A, C
Molecule details ›
Chains: A, C
Length: 256 amino acids
Theoretical weight: 28.95 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78536 (Residues: 219-474; Coverage: 32%)
Gene names: ADAM17, CSVP, TACE
Sequence domains: Metallo-peptidase family M12B Reprolysin-like
Structure domains: Collagenase (Catalytic Domain)
Disintegrin and metalloproteinase domain-containing protein 17 Chain: E
Molecule details ›
Chain: E
Length: 256 amino acids
Theoretical weight: 28.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78536 (Residues: 219-474; Coverage: 32%)
Gene names: ADAM17, CSVP, TACE
Sequence domains: Metallo-peptidase family M12B Reprolysin-like
Structure domains: Collagenase (Catalytic Domain)
Disintegrin and metalloproteinase domain-containing protein 17 Chain: I
Molecule details ›
Chain: I
Length: 256 amino acids
Theoretical weight: 28.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P78536 (Residues: 219-474; Coverage: 32%)
Gene names: ADAM17, CSVP, TACE
Sequence domains: Metallo-peptidase family M12B Reprolysin-like
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand INN 4 x INN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21
Unit cell:
a: 61.38Å b: 126.27Å c: 81.27Å
α: 90° β: 107.41° γ: 90°
R-values:
R R work R free
0.18 0.18 0.27
Expression system: Not provided

Quick links

Citations

40 review citations

The canonical Notch signaling pathway: unfolding the activation mechanism.
Kopan et al. (2009)
39 more

26 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
25 more