› 1c7p

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF MUTANT HUMAN LYSOZYME WITH FOUR EXTRA RESIDUES (EAEA) AT THE N-TERMINAL

Released:
DOI: 10.2210/pdb1c7p/pdb
PDB entry 1c7p coloured by chain, front view.
PDB entry 1c7p coloured by chain, side view.
PDB entry 1c7p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Effect of extra N-terminal residues on the stability and folding of human lysozyme expressed in Pichia pastoris.
Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K
Protein Eng 13 299-307 (2000)
PMID: 10810162

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 15.12 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P61626 (Residues: 15-148; Coverage: 100%)
Gene names: LYZ, LZM
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P6122
Unit cell:
a: 91.08Å b: 91.08Å c: 92.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 not available not available
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Developments in biotechnological production of sweet proteins.
Masuda et al. (2006)
22 other articles

2 mentions without citation

Evolutionary plasticity of protein families: coupling between sequence and structure variation.
Panchenko et al. (2005)
1 more