› 1c7u

Solution NMR

Complex of the DNA binding core domain of the transcription factor MEF2A with a 20mer oligonucleotide

Released:
DOI: 10.2210/pdb1c7u/pdb
PDB entry 1c7u coloured by chain, front view.
PDB entry 1c7u coloured by chain, side view.
PDB entry 1c7u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the MEF2A-DNA complex: structural basis for the modulation of DNA bending and specificity by MADS-box transcription factors.
Huang K, Louis JM, Donaldson L, Lim FL, Sharrocks AD, Clore GM
EMBO J 19 2615-28 (2000)
PMID: 10835359

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113528 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Myocyte-specific enhancer factor 2A Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 10.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02078 (Residues: 2-86; Coverage: 17%)
Gene names: MEF2, MEF2A
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
5'-D(*CP*TP*CP*GP*GP*CP*TP*AP*TP*TP*AP*AP*TP*AP*GP*CP*CP*GP*AP*G)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

MIKC-type MADS-domain proteins: structural modularity, protein interactions and network evolution in land plants.
Kaufmann et al. (2005)
6 more

6 mentions without citation

Solution structure of the MEF2A-DNA complex: structural basis for the modulation of DNA bending and specificity by MADS-box transcription factors.
Huang et al. (2000)
5 more