› 1c9b

X-ray diffraction
2.65Å resolution

CRYSTAL STRUCTURE OF A HUMAN TBP CORE DOMAIN-HUMAN TFIIB CORE DOMAIN COMPLEX BOUND TO AN EXTENDED, MODIFIED ADENOVIRAL MAJOR LATE PROMOTER (ADMLP)

Released:
DOI: 10.2210/pdb1c9b/pdb
PDB entry 1c9b coloured by chain, front view.
PDB entry 1c9b coloured by chain, side view.
PDB entry 1c9b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of preinitiation complex assembly on human pol II promoters.
Tsai FT, Sigler PB
EMBO J 19 25-36 (2000)
PMID: 10619841

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113532 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription initiation factor IIB Chains: A, E, I, M, Q
Molecule details ›
Chains: A, E, I, M, Q
Length: 207 amino acids
Theoretical weight: 23.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00403 (Residues: 110-316; Coverage: 66%)
Gene names: GTF2B, TF2B, TFIIB
Sequence domains: Transcription factor TFIIB repeat
Structure domains: Cyclin-like
TATA-box-binding protein Chains: B, F, J, N, R
Molecule details ›
Chains: B, F, J, N, R
Length: 180 amino acids
Theoretical weight: 20.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20226 (Residues: 158-337; Coverage: 53%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
ADMLP TATA-BOX DNA CONTAINING IIB RECOGNITION ELEMENT Chains: C, G, K, O, S
Molecule details ›
Chains: C, G, K, O, S
Length: 18 nucleotides
Theoretical weight: 5.63 KDa
ADMLP TATA-BOX DNA CONTAINING IIB RECOGNITION ELEMENT Chains: D, H, L, P, T
Molecule details ›
Chains: D, H, L, P, T
Length: 18 nucleotides
Theoretical weight: 5.4 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P21
Unit cell:
a: 118.45Å b: 122.298Å c: 140.217Å
α: 90° β: 113.08° γ: 90°
R-values:
R R work R free
0.237 0.229 0.26
Expression system: Escherichia coli

Quick links

Citations

20 review citations

The RNA polymerase II core promoter.
Smale et al. (2003)
19 more

22 mentions without citation

Cryo-EM in the study of challenging systems: the human transcription pre-initiation complex.
Nogales et al. (2016)
21 more