Function and Biology Details
Reaction catalysed:
Inactivates bleomycin B2 (a cytotoxic glycometallopeptide) by hydrolysis of a carboxyamide bond of beta-aminoalanine, but also shows general aminopeptidase activity. The specificity varies somewhat with source, but amino acid arylamides of Met, Leu and Ala are preferred (1).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
homo hexamer (preferred)
Assembly name:
Bleomycin hydrolase (preferred)
PDBe Complex ID:
PDB-CPX-171827 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bleomycin hydrolase
Molecule details ›
Chains: A, B, C
Length: 453 amino acids
Theoretical weight: 52.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Peptidase C1-like family
Structure domains: Cysteine proteinases
Length: 453 amino acids
Theoretical weight: 52.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q13867 (Residues: 2-454; Coverage: 100%)
Sequence domains: Peptidase C1-like family
Structure domains: Cysteine proteinases
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X26C
Spacegroup:
C2221
Expression system: Escherichia coli
