1cdw

X-ray diffraction
1.9Å resolution

HUMAN TBP CORE DOMAIN COMPLEXED WITH DNA

Released:
Source organism: Homo sapiens
Primary publication:
Crystal structure of a human TATA box-binding protein/TATA element complex.
Proc Natl Acad Sci U S A 93 4862-7 (1996)
PMID: 8643494

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-113536 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TATA-box-binding protein Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20226 (Residues: 159-337; Coverage: 53%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
DNA (5'-D(*CP*TP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*CP*TP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*AP*GP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Unit cell:
a: 45.8Å b: 78Å c: 97.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.258
Expression systems:
  • Escherichia coli
  • Not provided