› 1ch4

X-ray diffraction
2.5Å resolution

MODULE-SUBSTITUTED CHIMERA HEMOGLOBIN BETA-ALPHA (F133V)

Released:
DOI: 10.2210/pdb1ch4/pdb
PDB entry 1ch4 coloured by chain, front view.
PDB entry 1ch4 coloured by chain, side view.
PDB entry 1ch4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a protein with an artificial exon-shuffling, module M4-substituted chimera hemoglobin beta alpha, at 2.5 A resolution.
Shirai T, Fujikake M, Yamane T, Inaba K, Ishimori K, Morishima I
J Mol Biol 287 369-82 (1999)
PMID: 10080899

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hemoglobin subunit beta Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 146 amino acids
Theoretical weight: 15.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P68871 (Residues: 2-146; Coverage: 99%)
Gene name: HBB
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

2 bound ligands:
Ligand HEM 4 x HEM
Ligand CMO 4 x CMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 62.87Å b: 81.31Å c: 55.11Å
α: 90° β: 90.96° γ: 90°
R-values:
R R work R free
0.188 0.188 0.244
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Construction of heme enzymes: four approaches.
Watanabe Y. (2002)
4 other articles

3 mentions without citation

The RCSB protein data bank: integrative view of protein, gene and 3D structural information.
Rose et al. (2017)
2 more