› 1cjf

X-ray diffraction
2.3Å resolution

PROFILIN BINDS PROLINE-RICH LIGANDS IN TWO DISTINCT AMIDE BACKBONE ORIENTATIONS

Released:
DOI: 10.2210/pdb1cjf/pdb
PDB entry 1cjf coloured by chain, front view.
PDB entry 1cjf coloured by chain, side view.
PDB entry 1cjf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Profilin binds proline-rich ligands in two distinct amide backbone orientations.
Mahoney NM, Rozwarski DA, Fedorov E, Fedorov AA, Almo SC
Nat Struct Biol 6 666-71 (1999)
PMID: 10404225

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139733 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Profilin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 14.94 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P07737 (Residues: 2-140; Coverage: 99%)
Gene name: PFN1
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic
PROTEIN (PROLINE PEPTIDE) Chains: C, D
Molecule details ›
Chains: C, D
Length: 15 amino acids
Theoretical weight: 1.47 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand HOM 2 x HOM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 105.4Å b: 51.8Å c: 77.5Å
α: 90° β: 131.1° γ: 90°
R-values:
R R work R free
0.195 0.195 0.293
Expression system: Not provided

Quick links

Citations

11 review citations

The role of profilin complexes in cell motility and other cellular processes.
Witke W. (2004)
10 more

8 mentions without citation

Specificity and versatility of SH3 and other proline-recognition domains: structural basis and implications for cellular signal transduction.
Li SS. (2005)
7 more