› 1cjy

X-ray diffraction
2.5Å resolution

HUMAN CYTOSOLIC PHOSPHOLIPASE A2

Released:
DOI: 10.2210/pdb1cjy/pdb
PDB entry 1cjy coloured by chain, front view.
PDB entry 1cjy coloured by chain, side view.
PDB entry 1cjy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism.
Dessen A, Tang J, Schmidt H, Stahl M, Clark JD, Seehra J, Somers WS
Cell 97 349-60 (1999)
PMID: 10319815

Function and Biology Details

Reactions catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
2-lysophosphatidylcholine + H(2)O = glycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic phospholipase A2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 749 amino acids
Theoretical weight: 85.31 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P47712 (Residues: 1-749; Coverage: 100%)
Gene names: CPLA2, PLA2G4, PLA2G4A
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand MES 2 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 153.59Å b: 95.49Å c: 139.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.298
Expression system: Cricetulus griseus

Quick links

Citations

54 review citations

The expanding superfamily of phospholipase A(2) enzymes: classification and characterization.
Six et al. (2000)
53 more

41 mentions without citation

Using hydrogen/deuterium exchange mass spectrometry to define the specific interactions of the phospholipase A2 superfamily with lipid substrates, inhibitors, and membranes.
Cao et al. (2013)
40 more