› 1cqg

Solution NMR

HIGH RESOLUTION SOLUTION NMR STRUCTURE OF MIXED DISULFIDE INTERMEDIATE BETWEEN HUMAN THIOREDOXIN (C35A, C62A, C69A, C73A) MUTANT AND A 13 RESIDUE PEPTIDE COMPRISING ITS TARGET SITE IN HUMAN REF-1 (RESIDUES 59-71 OF THE P50 SUBUNIT OF NFKB), NMR, 31 STRUCTURES

Released:
DOI: 10.2210/pdb1cqg/pdb
PDB entry 1cqg coloured by chain, ensemble of 35 models, front view.
PDB entry 1cqg coloured by chain, ensemble of 35 models, side view.
PDB entry 1cqg coloured by chain, ensemble of 35 models, top view.
Source organism: Homo sapiens
Primary publication:
The solution structure of human thioredoxin complexed with its target from Ref-1 reveals peptide chain reversal.
Qin J, Clore GM, Kennedy WP, Kuszewski J, Gronenborn AM
Structure 4 613-20 (1996)
PMID: 8736558

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145350 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thioredoxin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.59 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10599 (Residues: 1-105; Coverage: 100%)
Gene names: TRDX, TRX, TRX1, TXN
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin
DNA repair nuclease/redox regulator APEX1, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P27695 (Residues: 59-71; Coverage: 4%)
Gene names: APE, APE1, APEX, APEX1, APX, HAP1, REF1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression system: Not provided

Quick links

Citations

20 review citations

Recent trends in glutathione biochemistry--glutathione-protein interactions: a molecular link between oxidative stress and cell proliferation?
Cotgreave et al. (1998)
19 more

6 mentions without citation

Amyotrophic lateral sclerosis: update and new developments.
Pratt et al. (2012)
5 more