› 1cw3

X-ray diffraction
2.58Å resolution

HUMAN MITOCHONDRIAL ALDEHYDE DEHYDROGENASE COMPLEXED WITH NAD+ AND MN2+

Released:
DOI: 10.2210/pdb1cw3/pdb
PDB entry 1cw3 coloured by chain, front view.
PDB entry 1cw3 coloured by chain, side view.
PDB entry 1cw3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human liver mitochondrial aldehyde dehydrogenase: three-dimensional structure and the restoration of solubility and activity of chimeric forms.
Ni L, Zhou J, Hurley TD, Weiner H
Protein Sci 8 2784-90 (1999)
PMID: 10631996

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 494 amino acids
Theoretical weight: 53.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05091 (Residues: 24-517; Coverage: 96%)
Gene names: ALDH2, ALDM
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
2 bound ligands:
Ligand MN 8 x MN
Ligand MG 8 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 101.4Å b: 176.3Å c: 102Å
α: 90° β: 94.7° γ: 90°
R-values:
R R work R free
0.18 0.175 0.242
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Targeting aldehyde dehydrogenase 2: new therapeutic opportunities.
Chen et al. (2014)
4 more

14 mentions without citation

Alda-1 is an agonist and chemical chaperone for the common human aldehyde dehydrogenase 2 variant.
Perez-Miller et al. (2010)
13 more