› 1cws

X-ray diffraction
2Å resolution

HUMAN CDC25B CATALYTIC DOMAIN WITH TUNGSTATE

Released:
DOI: 10.2210/pdb1cws/pdb
PDB entry 1cws coloured by chain, front view.
PDB entry 1cws coloured by chain, side view.
PDB entry 1cws coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the catalytic subunit of Cdc25B required for G2/M phase transition of the cell cycle.
Reynolds RA, Yem AW, Wolfe CL, Deibel MR, Chidester CG, Watenpaugh KD
J Mol Biol 293 559-68 (1999)
PMID: 10543950

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
M-phase inducer phosphatase 2 Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 24.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30305 (Residues: 370-580; Coverage: 36%)
Gene names: CDC25B, CDC25HU2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

4 bound ligands:
Ligand WO4 1 x WO4
Ligand SO4 1 x SO4
Ligand CL 1 x CL
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS
Spacegroup: P212121
Unit cell:
a: 51.734Å b: 71.863Å c: 74.935Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 not available not available
Expression system: Escherichia coli

Quick links

Citations

30 review citations

Cdc25 phosphatases and cancer.
Kristjánsdóttir et al. (2004)
29 more

3 mentions without citation

A stochastic context free grammar based framework for analysis of protein sequences.
Dyrka et al. (2009)
2 more