› 1dfc

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF HUMAN FASCIN, AN ACTIN-CROSSLINKING PROTEIN

Released:
DOI: 10.2210/pdb1dfc/pdb
PDB entry 1dfc coloured by chain, front view.
PDB entry 1dfc coloured by chain, side view.
PDB entry 1dfc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure, evolutionary conservation, and conformational dynamics of Homo sapiens fascin-1, an F-actin crosslinking protein.
Sedeh RS, Fedorov AA, Fedorov EV, Ono S, Matsumura F, Almo SC, Bathe M
J Mol Biol 400 589-604 (2010)
PMID: 20434460

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fascin Chains: A, B
Molecule details ›
Chains: A, B
Length: 493 amino acids
Theoretical weight: 54.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16658 (Residues: 1-493; Coverage: 100%)
Gene names: FAN1, FSCN1, HSN, SNL
Sequence domains: Fascin domain
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 165.426Å b: 71.689Å c: 116.924Å
α: 90° β: 132.17° γ: 90°
R-values:
R R work R free
0.184 0.184 0.268
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Actin dynamics, architecture, and mechanics in cell motility.
Blanchoin et al. (2014)
12 more

15 mentions without citation

Fascin-1: Updated biological functions and therapeutic implications in cancer biology.
Li et al. (2022)
14 more