› 1dpf

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF A MG-FREE FORM OF RHOA COMPLEXED WITH GDP

Released:
DOI: 10.2210/pdb1dpf/pdb
PDB entry 1dpf coloured by chain, front view.
PDB entry 1dpf coloured by chain, side view.
PDB entry 1dpf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An open conformation of switch I revealed by the crystal structure of a Mg2+-free form of RHOA complexed with GDP. Implications for the GDP/GTP exchange mechanism.
Shimizu T, Ihara K, Maesaki R, Kuroda S, Kaibuchi K, Hakoshima T
J Biol Chem 275 18311-7 (2000)
PMID: 10748207

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transforming protein RhoA Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 1-180; Coverage: 93%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-C
Spacegroup: P43212
Unit cell:
a: 91.2Å b: 91.2Å c: 56.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.252
Expression system: Escherichia coli

Quick links

Citations

4 review citations

GEF means go: turning on RHO GTPases with guanine nucleotide-exchange factors.
Rossman et al. (2005)
3 more

7 mentions without citation

Loss of col8a1a function during zebrafish embryogenesis results in congenital vertebral malformations.
Gray et al. (2014)
6 more