› 1drf

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE

Released:
DOI: 10.2210/pdb1drf/pdb
PDB entry 1drf coloured by chain, front view.
PDB entry 1drf coloured by chain, side view.
PDB entry 1drf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human dihydrofolate reductase complexed with folate.
Oefner C, D'Arcy A, Winkler FK
Eur J Biochem 174 377-85 (1988)
PMID: 3383852

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P00374 (Residues: 2-187; Coverage: 100%)
Gene name: DHFR
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand FOL 1 x FOL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R3
Unit cell:
a: 86.29Å b: 86.29Å c: 76.98Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 not available not available
Expression system: Not provided

Quick links

Citations

9 review citations

The methotrexate story: a paradigm for development of cancer chemotherapeutic agents.
Huennekens FM. (1994)
8 more

22 mentions without citation

Deamidation of human proteins.
Robinson et al. (2001)
21 more