› 1dyt

X-ray diffraction
1.75Å resolution

X-ray crystal structure of ECP (RNase 3) at 1.75 A

Released:
DOI: 10.2210/pdb1dyt/pdb
PDB entry 1dyt coloured by chain, front view.
PDB entry 1dyt coloured by chain, side view.
PDB entry 1dyt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Three-dimensional crystal structure of human eosinophil cationic protein (RNase 3) at 1.75 A resolution.
Mallorquí-Fernández G, Pous J, Peracaula R, Aymamí J, Maeda T, Tada H, Yamada H, Seno M, de Llorens R, Gomis-Rüth FX, Coll M
J Mol Biol 300 1297-307 (2000)
PMID: 10903870

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Eosinophil cationic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12724 (Residues: 28-160; Coverage: 100%)
Gene names: ECP, RNASE3, RNS3
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand FE 2 x FE
Ligand CIT 4 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P4322
Unit cell:
a: 62.163Å b: 62.163Å c: 174.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Mammalian antimicrobial proteins and peptides: overview on the RNase A superfamily members involved in innate host defence.
Boix et al. (2007)
23 other articles

13 mentions without citation

Using NMR spectroscopy to elucidate the role of molecular motions in enzyme function.
Lisi et al. (2016)
12 more