› 1e2s

X-ray diffraction
2.35Å resolution

Crystal structure of an Arylsulfatase A mutant C69A

Released:
DOI: 10.2210/pdb1e2s/pdb
PDB entry 1e2s coloured by chain, front view.
PDB entry 1e2s coloured by chain, side view.
PDB entry 1e2s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of an enzyme-substrate complex provides insight into the interaction between human arylsulfatase A and its substrates during catalysis.
von Bülow R, Schmidt B, Dierks T, von Figura K, Usón I
J Mol Biol 305 269-77 (2001)
PMID: 11124905

Function and Biology Details

Reaction catalysed: 
A cerebroside 3-sulfate + H(2)O = a cerebroside + sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Arylsulfatase A Chain: P
Molecule details ›
Chain: P
Length: 489 amino acids
Theoretical weight: 51.93 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P15289 (Residues: 19-507; Coverage: 100%)
Gene name: ARSA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand MG 1 x MG
Ligand CSN 1 x CSN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: I422
Unit cell:
a: 131.8Å b: 131.8Å c: 192.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.236
Expression system: Mus musculus

Quick links

Citations

13 review citations

Protein posttranslational modifications: the chemistry of proteome diversifications.
Walsh et al. (2005)
12 more

6 mentions without citation

Recon3D enables a three-dimensional view of gene variation in human metabolism.
Brunk et al. (2018)
5 more