› 1e3l

X-ray diffraction
2.5Å resolution

P47H mutant of mouse class II alcohol dehydrogenase complex with NADH

Released:
DOI: 10.2210/pdb1e3l/pdb
PDB entry 1e3l coloured by chain, front view.
PDB entry 1e3l coloured by chain, side view.
PDB entry 1e3l coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structures of mouse class II alcohol dehydrogenase reveal determinants of substrate specificity and catalytic efficiency.
Svensson S, Höög JO, Schneider G, Sandalova T
J Mol Biol 302 441-53 (2000)
PMID: 10970744

Function and Biology Details

Reaction catalysed: 
All-trans-retinol-[cellular-retinol-binding-protein] + NAD(+) = all-trans-retinal-[cellular-retinol-binding-protein] + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
All-trans-retinol dehydrogenase [NAD(+)] ADH4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 376 amino acids
Theoretical weight: 40.14 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QYY9 (Residues: 2-377; Coverage: 100%)
Gene names: Adh2, Adh4
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 79.156Å b: 80.639Å c: 102.757Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Ethanol's molecular targets.
Harris et al. (2008)
1 more

2 mentions without citation

Modeling of molecular interaction between apoptin, BCR-Abl and CrkL--an alternative approach to conventional rational drug design.
Panigrahi et al. (2012)
1 more