› 1e4k

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURE OF SOLUBLE HUMAN IGG1 FC FRAGMENT-FC-GAMMA RECEPTOR III COMPLEX

Released:
DOI: 10.2210/pdb1e4k/pdb
PDB entry 1e4k coloured by chain, front view.
PDB entry 1e4k coloured by chain, side view.
PDB entry 1e4k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The 3.2-A crystal structure of the human IgG1 Fc fragment-Fc gammaRIII complex.
Sondermann P, Huber R, Oosthuizen V, Jacob U
Nature 406 267-73 (2000)
PMID: 10917521

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130816 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 25.37 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 106-328; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Low affinity immunoglobulin gamma Fc region receptor III-B Chain: C
Molecule details ›
Chain: C
Length: 176 amino acids
Theoretical weight: 20.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75015 (Residues: 18-193; Coverage: 81%)
Gene names: CD16B, FCG3, FCGR3, FCGR3B, IGFR3
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GLA, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P6522
Unit cell:
a: 115.32Å b: 115.32Å c: 299.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.271 0.271 0.357
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

123 review citations

Fcgamma receptors as regulators of immune responses.
Nimmerjahn et al. (2008)
122 more

59 mentions without citation

IgG Fc engineering to modulate antibody effector functions.
Wang et al. (2018)
58 more