› 1e4x

X-ray diffraction
1.9Å resolution

crossreactive binding of a circularized peptide to an anti-TGFalpha antibody Fab-fragment

Released:
DOI: 10.2210/pdb1e4x/pdb
PDB entry 1e4x coloured by chain, front view.
PDB entry 1e4x coloured by chain, side view.
PDB entry 1e4x coloured by chain, top view.
Source organisms:
Primary publication:
Cross-reactive binding of cyclic peptides to an anti-TGFalpha antibody Fab fragment: an X-ray structural and thermodynamic analysis.
Hahn M, Winkler D, Welfle K, Misselwitz R, Welfle H, Wessner H, Zahn G, Scholz C, Seifert M, Harkins R, Schneider-Mergener J, Höhne W
J Mol Biol 314 293-309 (2001)
PMID: 11718562

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-219162 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
TAB2 Chain: H
Molecule details ›
Chain: H
Length: 217 amino acids
Theoretical weight: 23.35 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
TAB2 Chain: I
Molecule details ›
Chain: I
Length: 217 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Immunoglobulin kappa constant Chains: L, M
Molecule details ›
Chains: L, M
Length: 214 amino acids
Theoretical weight: 23.58 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P01837 (Residues: 1-107; Coverage: 100%)
Gene name: Igkc
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
CYCLIC PEPTIDE Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 7 amino acids
Theoretical weight: 818 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2
Unit cell:
a: 173.82Å b: 45.09Å c: 120.41Å
α: 90° β: 99.13° γ: 90°
R-values:
R R work R free
0.245 0.245 0.311
Expression system: Not provided

Quick links

Citations

4 review citations

Peptide arrays: from macro to micro.
Reimer et al. (2002)
3 more

4 mentions without citation

Blind prediction performance of RosettaAntibody 3.0: grafting, relaxation, kinematic loop modeling, and full CDR optimization.
Weitzner et al. (2014)
3 more