› 1e6i

X-ray diffraction
1.87Å resolution

Bromodomain from GCN5 complexed with acetylated H4 peptide

Released:
DOI: 10.2210/pdb1e6i/pdb
PDB entry 1e6i coloured by chain, front view.
PDB entry 1e6i coloured by chain, side view.
PDB entry 1e6i coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structural basis for the recognition of acetylated histone H4 by the bromodomain of histone acetyltransferase gcn5p.
Owen DJ, Ornaghi P, Yang JC, Lowe N, Evans PR, Ballario P, Neuhaus D, Filetici P, Travers AA
EMBO J 19 6141-9 (2000)
PMID: 11080160

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135460 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone acetyltransferase GCN5 Chain: A
Molecule details ›
Chain: A
Length: 121 amino acids
Theoretical weight: 14.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03330 (Residues: 320-439; Coverage: 27%)
Gene names: ADA4, GCN5, SWI9, YGR252W
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H4 Chain: P
Molecule details ›
Chain: P
Length: 15 amino acids
Theoretical weight: 1.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P02309 (Residues: 16-30; Coverage: 15%)
Gene names: HHF1, HHF2, N2752, YBR009C, YBR0122, YNL030W

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 1 x ALY

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 43.68Å b: 71.92Å c: 89.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.209
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

80 review citations

Functions of site-specific histone acetylation and deacetylation.
Shahbazian et al. (2007)
79 more

25 mentions without citation

How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers.
Taverna et al. (2007)
24 more