› 1e7k

X-ray diffraction
2.9Å resolution

Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment

Released:
DOI: 10.2210/pdb1e7k/pdb
PDB entry 1e7k coloured by chain, front view.
PDB entry 1e7k coloured by chain, side view.
PDB entry 1e7k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment.
Vidovic I, Nottrott S, Hartmuth K, Lührmann R, Ficner R
Mol Cell 6 1331-42 (2000)
PMID: 11163207

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157309 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
NHP2-like protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 128 amino acids
Theoretical weight: 14.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55769 (Residues: 1-128; Coverage: 100%)
Gene names: NHP2L1, SNU13
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
RNA (5'-R(*GP*CP*CP*AP*AP*UP*GP*AP*GP*GP*UP*UP*UP* AP*UP*CP*CP*GP*AP*GP*G*C(-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 7.07 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 45.756Å b: 55.287Å c: 146.735Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.302
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

33 review citations

Small nucleolar RNAs: an abundant group of noncoding RNAs with diverse cellular functions.
Kiss T. (2002)
32 more

23 mentions without citation

The Kink Turn, a Key Architectural Element in RNA Structure.
Huang et al. (2016)
22 more