› 1eaz

X-ray diffraction
1.4Å resolution

Crystal structure of the phosphoinositol (3,4)-bisphosphate binding PH domain of TAPP1 from human.

Released:
DOI: 10.2210/pdb1eaz/pdb
PDB entry 1eaz coloured by chain, front view.
PDB entry 1eaz coloured by chain, side view.
PDB entry 1eaz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the phosphatidylinositol 3,4-bisphosphate-binding pleckstrin homology (PH) domain of tandem PH-domain-containing protein 1 (TAPP1): molecular basis of lipid specificity.
Thomas CC, Dowler S, Deak M, Alessi DR, van Aalten DM
Biochem J 358 287-94 (2001)
PMID: 11513726

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pleckstrin homology domain-containing family A member 1 Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 14.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HB21 (Residues: 181-303; Coverage: 30%)
Gene names: PLEKHA1, TAPP1
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 65.017Å b: 102.796Å c: 41.471Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 not available 0.228
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Phosphoinositide recognition domains.
Lemmon MA. (2003)
13 more

11 mentions without citation

Structural bioinformatics prediction of membrane-binding proteins.
Bhardwaj et al. (2006)
10 more