› 1eb3

X-ray diffraction
1.75Å resolution

YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE 4,7-DIOXOSEBACIC ACID COMPLEX

Released:
DOI: 10.2210/pdb1eb3/pdb
PDB entry 1eb3 coloured by chain, front view.
PDB entry 1eb3 coloured by chain, side view.
PDB entry 1eb3 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The X-ray structure of yeast 5-aminolaevulinic acid dehydratase complexed with two diacid inhibitors.
Erskine PT, Coates L, Newbold R, Brindley AA, Stauffer F, Wood SP, Warren MJ, Cooper JB, Shoolingin-Jordan PM, Neier R
FEBS Lett 503 196-200 (2001)
PMID: 11513881

Function and Biology Details

Reaction catalysed: 
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138644 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-aminolevulinic acid dehydratase Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P05373 (Residues: 1-340; Coverage: 99%)
Gene names: HEM2, YGL040C
Sequence domains: Delta-aminolevulinic acid dehydratase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand DSB 1 x DSB
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: I422
Unit cell:
a: 104.3Å b: 104.3Å c: 167.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 not available 0.308
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The biochemistry of heme biosynthesis.
Heinemann et al. (2008)
2 more

4 mentions without citation

Structural relationships among proteins with different global topologies and their implications for function annotation strategies.
Petrey et al. (2009)
3 more