› 1efh

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE HUMAN HYDROXYSTEROID SULFOTRANSFERASE IN THE PRESENCE OF PAP

Released:
DOI: 10.2210/pdb1efh/pdb
PDB entry 1efh coloured by chain, front view.
PDB entry 1efh coloured by chain, side view.
PDB entry 1efh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of SULT2A3, human hydroxysteroid sulfotransferase.
Pedersen LC, Petrotchenko EV, Negishi M
FEBS Lett 475 61-4 (2000)
PMID: 10854859

Function and Biology Details

Reactions catalysed: 
3'-phosphoadenylyl sulfate + glycolithocholate = adenosine 3',5'-bisphosphate + glycolithocholate 3-sulfate
3'-phosphoadenylyl sulfate + an alcohol = adenosine 3',5'-bisphosphate + an alkyl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 2A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 292 amino acids
Theoretical weight: 34.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06520 (Residues: 1-285; Coverage: 100%)
Gene names: HST, STD, SULT2A1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand A3P 2 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P212121
Unit cell:
a: 73.19Å b: 96.82Å c: 127.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.239
Expression system: Escherichia coli

Quick links

Citations

11 review citations

A proposed nomenclature system for the cytosolic sulfotransferase (SULT) superfamily.
Blanchard et al. (2004)
10 more

23 mentions without citation

Regioselective sulfation and glucuronidation of phenolics: insights into the structural basis.
Wu et al. (2011)
22 more