› 1ek5

X-ray diffraction
1.8Å resolution

STRUCTURE OF HUMAN UDP-GALACTOSE 4-EPIMERASE IN COMPLEX WITH NAD+

Released:
DOI: 10.2210/pdb1ek5/pdb
PDB entry 1ek5 coloured by chain, front view.
PDB entry 1ek5 coloured by chain, side view.
PDB entry 1ek5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic evidence for Tyr 157 functioning as the active site base in human UDP-galactose 4-epimerase.
Thoden JB, Wohlers TM, Fridovich-Keil JL, Holden HM
Biochemistry 39 5691-701 (2000)
PMID: 10801319

Function and Biology Details

Reactions catalysed: 
UDP-alpha-D-glucose = UDP-alpha-D-galactose
UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-galactosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glucose 4-epimerase Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 38.32 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q14376 (Residues: 1-348; Coverage: 100%)
Gene name: GALE
Sequence domains: GDP-mannose 4,6 dehydratase
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 1 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 50.3Å b: 116.4Å c: 130.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.223
Expression system: Saccharomyces cerevisiae

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Citations

12 review citations

Medium- and short-chain dehydrogenase/reductase gene and protein families : the SDR superfamily: functional and structural diversity within a family of metabolic and regulatory enzymes.
Kavanagh et al. (2008)
11 more

4 mentions without citation

Gene expression patterns and catalytic properties of UDP-D-glucose 4-epimerases from barley (Hordeum vulgare L.).
Zhang et al. (2006)
3 more