› 1eku

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF A BIOLOGICALLY ACTIVE SINGLE CHAIN MUTANT OF HUMAN IFN-GAMMA

Released:
DOI: 10.2210/pdb1eku/pdb
PDB entry 1eku coloured by chain, front view.
PDB entry 1eku coloured by chain, side view.
PDB entry 1eku coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Design, characterization, and structure of a biologically active single-chain mutant of human IFN-gamma.
Landar A, Curry B, Parker MH, DiGiacomo R, Indelicato SR, Nagabhushan TL, Rizzi G, Walter MR
J Mol Biol 299 169-79 (2000)
PMID: 10860730

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interferon gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 31.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01579 (Residues: 24-144, 26-161; Coverage: 97%)
Gene name: IFNG
Sequence domains: Interferon gamma
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

1 bound ligand:
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R32
Unit cell:
a: 111.38Å b: 111.38Å c: 311.96Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.251 0.246 0.265
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Interferons, interferon-like cytokines, and their receptors.
Pestka et al. (2004)
4 more

6 mentions without citation

EPSVR and EPMeta: prediction of antigenic epitopes using support vector regression and multiple server results.
Liang et al. (2010)
5 more